Oligo Synthesis

In 1998 Wengel and co-workers described constrained nucleotides. They described it as a original class of conformationally limited oligonucleotide analogues. It is assumed as bicyclic nucleic acid where a ribonucleoside is closely connected among the 2'-oxygen and the 4'-carbon atoms all the way through a methylene unit.

Biosyn is competent enough to offer its valuable customers, various customized solutions for custom peptide synthesis, custom RNA synthesis, custom DNA synthesis, custom polyclonal antibody production, DNA real-time PCR products, DNA micro-array services, molecular diagnostic detection kits, DNA micro-array services and a wide range of custom DNA-based consumables and analytical kits since it is a market leader in genomic research and proteomic research products. 

As you know Bio-synthesis is exclusively located and able to synthesize long RNA segment with a bit longer in length, speedy turnaround period and one of the best quality.      

Oligonucleotides enclosed nucleotide show unparalleled thermal stability towards balancing RNA2 and DNA, which lets excellent unequal discrimination. Actually, the high binding similarity of constrained nucleotide oligo is prescribed for the purpose of any of the hydration assay that needs high specificity and reproducibility like:

Most of the constrained nucleotide oligo synthesis includes:

Ø  Preparation of mRNA sample

Ø  PCR primes and molecular beacons

Ø  Allele précised PNA

Ø  SNP genotyping

Ø  Analysis of DNA MicroArray expression

Ø  Tagged probes

Ø  In situ hybridization investigation

Ø  Capacities of multi scale              

Ø  Desalted, deprotected and prepared to use.

Ø  Quality analysis by MALDI-TOF Mass spectrometry, PAGE analysis and HPLC

Ø   High throughput capacity speedy capacity.

As a consequence of these important characteristics, the actual use of constrained nucleotide-altered oligo in antisense drug development is these days coming under investigation4 and, freshly, the therapeutic potential of constrained nucleotide has been evaluated. There is an contribution from BSI that is constrained nucleotide synthesis with great flexibility.

Quality that is fully guaranteed

Here at Bio-synthesis, we are committed to provide on our reputation for one of the best quality nucleic acid synthesis. As an important part of our long lasting assurance to the best quality, we implement the following standards for the assurance of quality:

DNA, RNA, PNA, siRNA, and all nucleic acid synthesis products are evaluated by mass spectrometry and quantified at least two times by uv spectrophotometry (unless no post-synthesis processing is demanded)

All standard RNA, DNA, PNA and siRNA synthesis requires MALDI-TOFMS.

ESI-MS for all extended or highly customized oligonucleotides.

PAGE, HPLC, RNase Free HPLC and PAGE for cleansing of RNA and DNA products.

CGE or UPLC for quality evaluation when suitable.           

Quality checking methods that are fully customized and documentation are available upon request.

Assured Quality with excellent features

At Bio-synthesis, we feel very proud on our old and good reputation for high and better quality synthesis of nucleic acid.

As an important part of long-standing promise to quality, we use the following standards for excellence pledge:

TOFMS-MALDI for every standard RNA, DNA, siRNA, and PNA synthesis

MS-ESI for every longer or highly customized oligonucleotides.

For more information on  Peptide Synthesis, Amino acid analysis,  Long RNA, Please Visit :  Oligo Synthesis